The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide Nucleic Acid Sequences

The implication of nanopores as versatile components in dedicated biosensors, nanoreactors, or miniaturized sequencers has considerably advanced single-molecule investigative science a wide range disciplines, ranging from molecular medicine and nanoscale chemistry to biophysics ecology. Here, we employed the nanopore tweezing technique capture amino acid-functionalized peptide nucleic acids (PNAs) with α-hemolysin-based correlated ensuing stochastic fluctuations ionic current through composition order bases PNAs primary structure. We demonstrated that while system enables detection distinct on homopolymeric PNA triplet heteropolymeric strands, it also reveals rich insights into conformational dynamics entrapped within nanopore, relevant for perfecting recognition capability sequencing.